6eni

X-ray diffraction
1.1Å resolution

Tryptophan Repressor TrpR from E.coli variant T44L S88Y with Indole-3-acetic acid as ligand

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Trp operon repressor Chains: A, B
Molecule details ›
Chains: A, B
Length: 116 amino acids
Theoretical weight: 13.53 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0A881 (Residues: 1-108; Coverage: 100%)
Gene names: JW4356, b4393, rtrY, trpR
Sequence domains: Trp repressor protein
Structure domains: TrpR-like

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 54.78Å b: 63.41Å c: 64.77Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.158 0.176
Expression system: Escherichia coli BL21