› 6emf

X-ray diffraction
2.65Å resolution

Crystal structure of Rrp1 from Chaetomium thermophilum in space group C2

Released:
DOI: 10.2210/pdb6emf/pdb
PDB 6emf coloured by chain and viewed from the front.
PDB 6emf coloured by chain and viewed from the side.
PDB 6emf coloured by chain and viewed from the top.
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Primary publication:
Visualizing the Assembly Pathway of Nucleolar Pre-60S Ribosomes.
Kater L, Thoms M, Barrio-Garcia C, Cheng J, Ismail S, Ahmed YL, Bange G, Kressler D, Berninghausen O, Sinning I, Hurt E, Beckmann R
Cell 171 1599-1610.e14 (2017)
PMID: 29245012

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
G0S4M2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 290 amino acids
Theoretical weight: 33.1 KDa
Source organism: Chaetomium thermophilum var. thermophilum DSM 1495
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G0S4M2 (Residues: 1-282; Coverage: 100%)
Gene name: CTHT_0031510
Sequence domains: Nucleolar protein,Nop52

Ligands and Environments

2 bound ligands:
Ligand EDO 7 x EDO
Ligand PRO 2 x PRO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 131.72Å b: 74.675Å c: 68.795Å
α: 90° β: 90.53° γ: 90°
R-values:
R R work R free
0.2 0.199 0.232
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Genetics animates structure: leveraging genetic interactions to study the dynamics of ribosome biogenesis.
Black et al. (2021)
10 more