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PRISEs (progesterone 5β-reductase and/or iridoid synthase-like 1,4-enone reductases): Catalytic and substrate promiscuity allows for realization of multiple pathways in plant metabolism.

Schmidt K, Petersen J, Munkert J, Egerer-Sieber C, Hornig M, Muller YA, Kreis W
Phytochemistry 156 9-19 (2018)
Cited: 7 times
EuropePMC logo PMID: 30172078

Abstract

PRISEs (progesterone 5β-reductase and/or iridoid synthase-like 1,4-enone reductases) are involved in cardenolide and iridoid biosynthesis. We here investigated a PRISE (rAtSt5βR) from Arabidopsis thaliana, a plant producing neither cardenolides nor iridoids. The structure of rAtSt5βR was elucidated with X-ray crystallography and compared to the known structures of PRISEs from Catharanthus roseus (rCrISY) and Digitalis lanata (rDlP5βR). The three enzymes show a high degree of sequence and structure conservation in the active site. Amino acids previously considered to allow discrimination between progesterone 5β-reductase and iridoid synthase were interchanged among rAtSt5βR, rCrISY and rDlP5βR applying site-directed mutagenesis. Structural homologous substitutions had different effects, and changes in progesterone 5β-reductase and iridoid synthase activity were not correlated in all cases. Our results help to explain fortuitous emergence of metabolic pathways and product accumulation. The fact that PRISEs are found ubiquitously in spermatophytes insinuates that PRISEs might have a more general function in plant metabolism such as, for example, the detoxification of reactive carbonyl species.

Articles - 6el3 mentioned but not cited (2)

  1. Biosynthetic approach to combine the first steps of cardenolide formation in Saccharomyces cerevisiae. Rieck C, Geiger D, Munkert J, Messerschmidt K, Petersen J, Strasser J, Meitinger N, Kreis W. Microbiologyopen 8 e925 (2019)
  2. Knockout of Arabidopsis thaliana VEP1, Encoding a PRISE (Progesterone 5β-Reductase/Iridoid Synthase-Like Enzyme), Leads to Metabolic Changes in Response to Exogenous Methyl Vinyl Ketone (MVK). Klein J, Ernst M, Christmann A, Tropper M, Leykauf T, Kreis W, Munkert J. Metabolites 12 11 (2021)


Reviews citing this publication (1)

  1. Using interdisciplinary, phylogeny-guided approaches to understand the evolution of plant metabolism. Schenck CA, Busta L. Plant Mol Biol 109 355-367 (2022)

Articles citing this publication (4)

  1. The Progesterone 5β-Reductase/Iridoid Synthase Family: A Catalytic Reservoir for Specialized Metabolism across Land Plants. Nguyen TD, O'Connor SE. ACS Chem Biol 15 1780-1787 (2020)
  2. Unveiling the genetic basis of Sclerotinia head rot resistance in sunflower. Filippi CV, Zubrzycki JE, Di Rienzo JA, Quiroz FJ, Puebla AF, Alvarez D, Maringolo CA, Escande AR, Hopp HE, Heinz RA, Paniego NB, Lia VV. BMC Plant Biol 20 322 (2020)
  3. RNAi-mediated gene knockdown of progesterone 5β-reductases in Digitalis lanata reduces 5β-cardenolide content. Klein J, Horn E, Ernst M, Leykauf T, Leupold T, Dorfner M, Wolf L, Ignatova A, Kreis W, Munkert J. Plant Cell Rep 40 1631-1646 (2021)
  4. Identification and functional characterization of three iridoid synthases in Gardenia jasminoides. Xu C, Ye P, Wu Q, Liang S, Wei W, Yang J, Chen W, Zhan R, Ma D. Planta 255 58 (2022)


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