› 6ek9

X-ray diffraction
1.5Å resolution

Cytosolic copper storage protein Csp from Streptomyces lividans: Cu loaded form

Released:
DOI: 10.2210/pdb6ek9/pdb
PDB 6ek9 coloured by chain and viewed from the front.
PDB 6ek9 coloured by chain and viewed from the side.
PDB 6ek9 coloured by chain and viewed from the top.
Source organism: Streptomyces lividans
Primary publication:
A cytosolic copper storage protein provides a second level of copper tolerance in Streptomyces lividans.
Straw ML, Chaplin AK, Hough MA, Paps J, Bavro VN, Wilson MT, Vijgenboom E, Worrall JAR
Metallomics 10 180-193 (2018)
PMID: 29292456

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Four-helix bundle copper-binding protein Chain: A
Molecule details ›
Chain: A
Length: 121 amino acids
Theoretical weight: 12.62 KDa
Source organism: Streptomyces lividans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D6ES11 (Residues: 16-136; Coverage: 89%)
Gene name: SSPG_04277
Sequence domains: Domain of Unknown Function (DUF326)

Ligands and Environments

1 bound ligand:
Ligand CU1 20 x CU1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: I222
Unit cell:
a: 62.081Å b: 64.138Å c: 66.003Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.206 0.231
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Unique underlying principles shaping copper homeostasis networks.
Novoa-Aponte et al. (2022)
3 more