6ehs

X-ray diffraction
1.5Å resolution

E. coli Hydrogenase-2 chemically reduced structure

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Hydrogenase (acceptor) Chains: S, T
Molecule details ›
Chains: S, T
Length: 300 amino acids
Theoretical weight: 32.63 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: B7LGE4 (Residues: 38-330; Coverage: 79%)
Gene names: EC55989_3414, hybO
Sequence domains:
Structure domains:
Hydrogenase-2 large chain Chains: L, M
Molecule details ›
Chains: L, M
Length: 552 amino acids
Theoretical weight: 61.05 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ACE1 (Residues: 1-552; Coverage: 97%)
Gene names: ECs3879, Z4348, hybC
Sequence domains: Nickel-dependent hydrogenase
Structure domains: Cytochrome-c3 Hydrogenase, chain B

Ligands and Environments

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 99.885Å b: 100.065Å c: 168.801Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.126 0.124 0.163
Expression system: Escherichia coli