6eg8

X-ray diffraction
2.8Å resolution

Structure of the GDP-bound Gs heterotrimer

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chains: A, B, D, F
Molecule details ›
Chains: A, B, D, F
Length: 345 amino acids
Theoretical weight: 37.73 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chains: C, E, G, H
Molecule details ›
Chains: C, E, G, H
Length: 71 amino acids
Theoretical weight: 7.85 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chains: I, J, K, L
Molecule details ›
Chains: I, J, K, L
Length: 381 amino acids
Theoretical weight: 44.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63092 (Residues: 4-394; Coverage: 96%)
  • Best match: P63092-3 (Residues: 4-71, 72-379)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 100.86Å b: 100.81Å c: 179.33Å
α: 90° β: 106.18° γ: 90°
R-values:
R R work R free
0.218 0.217 0.269
Expression systems:
  • Trichoplusia ni
  • Escherichia coli