› 6efv

X-ray diffraction
2.34Å resolution

The NADPH-dependent sulfite reductase flavoprotein adopts an extended conformation that is unique to this diflavin reductase

Released:
DOI: 10.2210/pdb6efv/pdb
PDB entry 6efv coloured by chain, front view.
PDB entry 6efv coloured by chain, side view.
PDB entry 6efv coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
NADPH-dependent sulfite reductase flavoprotein adopts an extended conformation unique to this diflavin reductase.
Tavolieri AM, Murray DT, Askenasy I, Pennington JM, McGarry L, Stanley CB, Stroupe ME
J Struct Biol 205 170-179 (2019)
PMID: 30654136

Function and Biology Details

Reaction catalysed: 
H(2)S + 3 NADP(+) + 3 H(2)O = sulfite + 3 NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197807 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sulfite reductase [NADPH] flavoprotein alpha-component Chain: A
Molecule details ›
Chain: A
Length: 569 amino acids
Theoretical weight: 63.61 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: W8SX42 (Residues: 64-599; Coverage: 89%)
Gene names: ACU57_24495, BGM66_004141, BJI68_01120, C0P57_002186, CG831_002173, CIG67_16065, CTR35_002148, E6D34_09095, EAN77_05440, G3V95_12665, G4A38_14970, G4A47_01900, GNW61_06245, GOP25_06565, GP965_11530, J0541_001304, JNP96_21415, KV499_000078, NCTC9045_01238, NCTC9706_03313, QDW62_05285, cysJ
Sequence domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD

Cofactor: Ligand FMN 1 x FMN
2 bound ligands:
Ligand SO4 6 x SO4
Ligand CXS 2 x CXS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P212121
Unit cell:
a: 60.483Å b: 99.739Å c: 103.533Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.167 0.234
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Metalloprotein catalysis: structural and mechanistic insights into oxidoreductases from neutron protein crystallography.
Schröder et al. (2021)
1 more

2 mentions without citation

Mining the Flavoproteome of Brucella ovis, the Brucellosis Causing Agent in Ovis aries.
Minjárez-Sáenz et al. (2022)
1 more