› 6ec7

X-ray diffraction
2.15Å resolution

Glutamylation domain, TbtB, from thiomuracin biosynthesis

Released:
DOI: 10.2210/pdb6ec7/pdb
PDB entry 6ec7 coloured by chain, front view.
PDB entry 6ec7 coloured by chain, side view.
PDB entry 6ec7 coloured by chain, top view.
Source organism: Thermobispora bispora DSM 43833
Primary publication:
Characterization of glutamyl-tRNA-dependent dehydratases using nonreactive substrate mimics.
Bothwell IR, Cogan DP, Kim T, Reinhardt CJ, van der Donk WA, Nair SK
Proc Natl Acad Sci U S A 116 17245-17250 (2019)
PMID: 31409709

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112833 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lantibiotic dehydratase N-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 861 amino acids
Theoretical weight: 96.01 KDa
Source organism: Thermobispora bispora DSM 43833
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D6Y502 (Residues: 1-858; Coverage: 100%)
Gene name: Tbis_0550
Sequence domains: Lantibiotic dehydratase, N terminus

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21
Unit cell:
a: 44.819Å b: 96.406Å c: 107.013Å
α: 90° β: 98.76° γ: 90°
R-values:
R R work R free
0.217 0.214 0.277
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

New developments in RiPP discovery, enzymology and engineering.
Montalbán-López et al. (2021)
1 more

2 mentions without citation

Characterization of glutamyl-tRNA-dependent dehydratases using nonreactive substrate mimics.
Bothwell et al. (2019)
1 more