› 6ebt

X-ray diffraction
2.3Å resolution

Crystal structure of recombinant mutant N107T of human fumarase

Released:
DOI: 10.2210/pdb6ebt/pdb
PDB 6ebt coloured by chain and viewed from the front.
PDB 6ebt coloured by chain and viewed from the side.
PDB 6ebt coloured by chain and viewed from the top.
Source organism: Homo sapiens
Entry authors: Ajalla MAA, Nonato MC

Function and Biology Details

Reaction catalysed: 
(S)-malate = fumarate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fumarate hydratase, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 466 amino acids
Theoretical weight: 50.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07954 (Residues: 45-510; Coverage: 91%)
Gene name: FH
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P6522
Unit cell:
a: 188.816Å b: 188.816Å c: 114.145Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.152 0.15 0.185
Expression system: Escherichia coli BL21(DE3)

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