6ebt

X-ray diffraction
2.3Å resolution

Crystal structure of recombinant mutant N107T of human fumarase

Released:
Source organism: Homo sapiens
Entry authors: Ajalla MAA, Nonato MC

Function and Biology Details

Reaction catalysed:
(S)-malate = fumarate + H(2)O
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fumarate hydratase, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 466 amino acids
Theoretical weight: 50.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07954 (Residues: 45-510; Coverage: 91%)
Gene name: FH
Sequence domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P6522
Unit cell:
a: 188.816Å b: 188.816Å c: 114.145Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.152 0.15 0.185
Expression system: Escherichia coli BL21(DE3)