6e97

X-ray diffraction
1.8Å resolution

Crystal structure of the aryl acid adenylating enzyme FscC from Fuscachelin NRPS in complex with DHB-adenylate

Released:
Source organism: Thermobifida fusca YX
Entry authors: Bruner SD, Zagulyaeva AA

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2,3-dihydroxybenzoate-AMP ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 546 amino acids
Theoretical weight: 59.75 KDa
Source organism: Thermobifida fusca YX
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q47NR5 (Residues: 1-543; Coverage: 100%)
Gene name: Tfu_1871
Sequence domains:

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: C2
Unit cell:
a: 206.127Å b: 51.327Å c: 111.363Å
α: 90° β: 119.88° γ: 90°
R-values:
R R work R free
0.194 0.184 0.217
Expression system: Escherichia coli BL21(DE3)