6e8o

X-ray diffraction
1.7Å resolution

Crystal structure of aryl acid adenylating enzyme FscC from Fuscachelin NRPS in complex with AMP

Released:
Source organism: Thermobifida fusca YX
Entry authors: Bruner SD, Zagulyaeva AA

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2,3-dihydroxybenzoate-AMP ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 546 amino acids
Theoretical weight: 59.75 KDa
Source organism: Thermobifida fusca YX
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q47NR5 (Residues: 1-543; Coverage: 100%)
Gene name: Tfu_1871
Sequence domains:

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: C2
Unit cell:
a: 207.034Å b: 51.022Å c: 111.552Å
α: 90° β: 119.87° γ: 90°
R-values:
R R work R free
0.196 0.189 0.216
Expression system: Escherichia coli BL21(DE3)