› 6e5t

X-ray diffraction
1.55Å resolution

Crystal structure of human cellular retinol binding protein 1 in complex with abnormal-cannabidiorcin (Abn-CBDO)

Released:
DOI: 10.2210/pdb6e5t/pdb
PDB entry 6e5t coloured by chain, front view.
PDB entry 6e5t coloured by chain, side view.
PDB entry 6e5t coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Abnormal Cannabidiol Modulates Vitamin A Metabolism by Acting as a Competitive Inhibitor of CRBP1.
Silvaroli JA, Widjaja-Adhi MAK, Trischman T, Chelstowska S, Horwitz S, Banerjee S, Kiser PD, Blaner WS, Golczak M
ACS Chem Biol 14 434-448 (2019)
PMID: 30721022

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140657 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Retinol-binding protein 1 Chain: A
Molecule details ›
Chain: A
Length: 140 amino acids
Theoretical weight: 16.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P09455 (Residues: 2-135; Coverage: 99%)
Gene names: CRBP1, RBP1
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin

Ligands and Environments

1 bound ligand:
Ligand HVJ 1 x HVJ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 37.319Å b: 40.739Å c: 92.079Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.176 0.224
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

The molecular aspects of absorption and metabolism of carotenoids and retinoids in vertebrates.
Widjaja-Adhi et al. (2020)
3 more

1 mention without citation

Abnormal Cannabidiol Modulates Vitamin A Metabolism by Acting as a Competitive Inhibitor of CRBP1.
Silvaroli et al. (2019)