› 6e4l

X-ray diffraction
1.6Å resolution

The structure of the N-terminal domain of human clathrin heavy chain 1 (nTD) in complex with ES9

Released:
DOI: 10.2210/pdb6e4l/pdb
PDB entry 6e4l coloured by chain, front view.
PDB entry 6e4l coloured by chain, side view.
PDB entry 6e4l coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Disruption of endocytosis through chemical inhibition of clathrin heavy chain function.
Dejonghe W, Sharma I, Denoo B, De Munck S, Lu Q, Mishev K, Bulut H, Mylle E, De Rycke R, Vasileva M, Savatin DV, Nerinckx W, Staes A, Drozdzecki A, Audenaert D, Yperman K, Madder A, Friml J, Van Damme D, Gevaert K, Haucke V, Savvides SN, Winne J, Russinova E
Nat Chem Biol 15 641-649 (2019)
PMID: 31011214

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169454 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Clathrin heavy chain 1 Chain: A
Molecule details ›
Chain: A
Length: 369 amino acids
Theoretical weight: 41.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q00610 (Residues: 1-364; Coverage: 22%)
Gene names: CLH17, CLTC, CLTCL2, KIAA0034
Sequence domains:
Structure domains: Clathrin heavy-chain terminal domain

Ligands and Environments

5 bound ligands:
Ligand DMS 2 x DMS
Ligand GOL 1 x GOL
Ligand EDO 2 x EDO
Ligand ACT 3 x ACT
Ligand HRS 1 x HRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P212121
Unit cell:
a: 71.21Å b: 74.34Å c: 84.23Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.188 0.214
Expression system: Escherichia coli

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Citations

11 review citations

Understanding nanoparticle endocytosis to improve targeting strategies in nanomedicine.
Sousa de Almeida et al. (2021)
10 more