› 6e4e

X-ray diffraction
1.9Å resolution

Crystal structure of dihydrofolate reductase from Staphylococcus aureus MW2 bound to NADP and p218

Released:
DOI: 10.2210/pdb6e4e/pdb
PDB entry 6e4e coloured by chain, front view.
PDB entry 6e4e coloured by chain, side view.
PDB entry 6e4e coloured by chain, top view.
Source organism: Staphylococcus aureus
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140887 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrofolate reductase Chain: A
Molecule details ›
Chain: A
Length: 182 amino acids
Theoretical weight: 20.74 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A017 (Residues: 1-159; Coverage: 100%)
Gene name: folA
Sequence domains: Dihydrofolate reductase
Structure domains: Dihydrofolate Reductase, subunit A

Ligands and Environments


Cofactor: Ligand NAP 1 x NAP
1 bound ligand:
Ligand MMV 1 x MMV
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P6122
Unit cell:
a: 79Å b: 79Å c: 108.93Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.18 0.224
Expression system: Escherichia coli

Quick links