PDBe 6e13

X-ray diffraction
2.35Å resolution

Pseudomonas putida PqqB with a non-physiological zinc at the active site binds the substrate mimic, 5-cysteinyl-3,4-dihydroxyphenylalanine (5-Cys-DOPA), non-specifically but supports the proposed function of the enzyme in pyrroloquinoline quinone biosynthesis.

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Coenzyme PQQ synthesis protein B Chain: A
Molecule details ›
Chain: A
Length: 308 amino acids
Theoretical weight: 34 KDa
Source organism: Pseudomonas putida KT2440
Expression system: Escherichia coli
UniProt:
  • Canonical: Q88QV5 (Residues: 1-303; Coverage: 100%)
Gene names: PP_0379, pqqB
Sequence domains: Beta-lactamase superfamily domain

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P43212
Unit cell:
a: 86.038Å b: 86.038Å c: 107.128Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.189 0.236
Expression system: Escherichia coli