› 6e0n

X-ray diffraction
1.5Å resolution

Structure of Elizabethkingia meningoseptica CdnE cyclic dinucleotide synthase with GTP and Apcpp

Released:
DOI: 10.2210/pdb6e0n/pdb
PDB 6e0n coloured by chain and viewed from the front.
PDB 6e0n coloured by chain and viewed from the side.
PDB 6e0n coloured by chain and viewed from the top.
Source organism: Elizabethkingia meningoseptica ATCC 13253 = NBRC 12535
Primary publication:
Bacterial cGAS-like enzymes synthesize diverse nucleotide signals.
Whiteley AT, Eaglesham JB, de Oliveira Mann CC, Morehouse BR, Lowey B, Nieminen EA, Danilchanka O, King DS, Lee ASY, Mekalanos JJ, Kranzusch PJ
OpenAccess logo Nature 567 194-199 (2019)
PMID: 30787435

Function and Biology Details

Reactions catalysed: 
2 ATP = 2 diphosphate + cyclic di-3',5'-adenylate
2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyclic dipurine nucleotide synthase Chain: A
Molecule details ›
Chain: A
Length: 292 amino acids
Theoretical weight: 34.2 KDa
Source organism: Elizabethkingia meningoseptica ATCC 13253 = NBRC 12535
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DSP2 (Residues: 1-292; Coverage: 100%)
Gene names: NCTC10016_00133, cdnE01
Sequence domains: Second Messenger Oligonucleotide or Dinucleotide Synthetase domain

Ligands and Environments

3 bound ligands:
Ligand APC 1 x APC
Ligand GTP 2 x GTP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 57.195Å b: 58.231Å c: 99.446Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.155 0.18
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Conserved strategies for pathogen evasion of cGAS-STING immunity.
Eaglesham et al. (2020)
9 more

1 mention without citation

Bacterial cGAS-like enzymes synthesize diverse nucleotide signals.
Whiteley et al. (2019)