› 6e07

X-ray diffraction
2.6Å resolution

Crystal structure of Canton G6PD in complex with structural NADP

Released:
DOI: 10.2210/pdb6e07/pdb
PDB entry 6e07 coloured by chain, front view.
PDB entry 6e07 coloured by chain, side view.
PDB entry 6e07 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Correcting glucose-6-phosphate dehydrogenase deficiency with a small-molecule activator.
Hwang S, Mruk K, Rahighi S, Raub AG, Chen CH, Dorn LE, Horikoshi N, Wakatsuki S, Chen JK, Mochly-Rosen D
Nat Commun 9 4045 (2018)
PMID: 30279493

Function and Biology Details

Reaction catalysed: 
D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145820 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose-6-phosphate 1-dehydrogenase Chains: B, C, F, L, N, Q, T, W
Molecule details ›
Chains: B, C, F, L, N, Q, T, W
Length: 515 amino acids
Theoretical weight: 59.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P11413 (Residues: 1-515; Coverage: 100%)
Gene name: G6PD
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAP 8 x NAP
2 bound ligands:
Ligand PO4 8 x PO4
Ligand GOL 29 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 127.12Å b: 206.25Å c: 211.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.187 0.211
Expression system: Escherichia coli K-12

Quick links

Citations

13 review citations

Treatment strategies for glucose-6-phosphate dehydrogenase deficiency: past and future perspectives.
Garcia et al. (2021)
12 more

5 mentions without citation

Correcting glucose-6-phosphate dehydrogenase deficiency with a small-molecule activator.
Hwang et al. (2018)
4 more