6dzt

Electron Microscopy
2.99Å resolution

Cryo-EM structure of nucleosome in complex with a single chain antibody fragment

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-207789 (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (7 distinct):
Histone H3 Chains: A, E
Molecule details ›
Chains: A, E
Length: 136 amino acids
Theoretical weight: 15.42 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
Histone H4 Chains: B, F
Molecule details ›
Chains: B, F
Length: 104 amino acids
Theoretical weight: 11.52 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
Histone H2A Chains: C, G
Molecule details ›
Chains: C, G
Length: 124 amino acids
Theoretical weight: 13.39 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
Histone H2B Chains: D, H
Molecule details ›
Chains: D, H
Length: 124 amino acids
Theoretical weight: 13.84 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
scFv Chains: M, N
Molecule details ›
Chains: M, N
Length: 265 amino acids
Theoretical weight: 29.03 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
DNA (147-MER) Chain: I
Molecule details ›
Chain: I
Length: 147 nucleotides
Theoretical weight: 45.61 KDa
Source organism: Escherichia coli
Expression system: Not provided
DNA (147-MER) Chain: J
Molecule details ›
Chain: J
Length: 147 nucleotides
Theoretical weight: 45.14 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.99Å
Relevant EMDB volumes: EMD-8938
Expression systems:
  • Escherichia coli
  • Not provided