› 6dyl

X-ray diffraction
1.69Å resolution

Vanadyl-bound structure of the engineered cyt b562 variant, CH3Y*

Released:
DOI: 10.2210/pdb6dyl/pdb
PDB entry 6dyl coloured by chain, front view.
PDB entry 6dyl coloured by chain, side view.
PDB entry 6dyl coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
An efficient, step-economical strategy for the design of functional metalloproteins.
Rittle J, Field MJ, Green MT, Tezcan FA
Nat Chem 11 434-441 (2019)
PMID: 30778140

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142149 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Soluble cytochrome b562 Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 106 amino acids
Theoretical weight: 11.93 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
Gene name: cybC
Sequence domains: Cytochrome b562

Ligands and Environments

1 bound ligand:
Ligand V 2 x V
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: C2221
Unit cell:
a: 49.031Å b: 98.405Å c: 178.753Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.187 0.226
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Protein Assembly by Design.
Zhu et al. (2021)
4 more

2 mentions without citation

Interaction of Vanadium Complexes with Proteins: Revisiting the Reported Structures in the Protein Data Bank (PDB) since 2015.
Santos et al. (2023)
1 more