› 6dyg

X-ray diffraction
1.49Å resolution

Fe(II)-bound structure of the engineered cyt cb562 variant, CH3Y

Released:
DOI: 10.2210/pdb6dyg/pdb
PDB entry 6dyg coloured by chain, front view.
PDB entry 6dyg coloured by chain, side view.
PDB entry 6dyg coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
An efficient, step-economical strategy for the design of functional metalloproteins.
Rittle J, Field MJ, Green MT, Tezcan FA
Nat Chem 11 434-441 (2019)
PMID: 30778140

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142149 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Soluble cytochrome b562 Chains: A, B
Molecule details ›
Chains: A, B
Length: 106 amino acids
Theoretical weight: 11.92 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
Gene name: cybC
Sequence domains: Cytochrome b562

Ligands and Environments

3 bound ligands:
Ligand HEC 2 x HEC
Ligand FE 1 x FE
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 34.739Å b: 83.513Å c: 39.088Å
α: 90° β: 99.99° γ: 90°
R-values:
R R work R free
0.192 0.19 0.217
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Protein Assembly by Design.
Zhu et al. (2021)
5 more

1 mention without citation

An efficient, step-economical strategy for the design of functional metalloproteins.
Rittle et al. (2019)