› 6dxs

X-ray diffraction
1.65Å resolution

Crystal structure of the LigJ hydratase E284Q mutant substrate complex with (3Z)-2-keto-4-carboxy-3-hexenedioate

Released:
DOI: 10.2210/pdb6dxs/pdb
PDB 6dxs coloured by chain and viewed from the front.
PDB 6dxs coloured by chain and viewed from the side.
PDB 6dxs coloured by chain and viewed from the top.
Source organism: Sphingobium sp. SYK-6
Primary publication:
Structure and Reaction Mechanism of the LigJ Hydratase: An Enzyme Critical for the Bacterial Degradation of Lignin in the Protocatechuate 4,5-Cleavage Pathway.
Hogancamp TN, Mabanglo MF, Raushel FM
Biochemistry 57 5841-5850 (2018)
PMID: 30207699

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-keto-4-carboxy-3-hexenedioate hydratase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 342 amino acids
Theoretical weight: 38.2 KDa
Source organism: Sphingobium sp. SYK-6
Expression system: Escherichia coli
UniProt:
  • Canonical: G2IQQ5 (Residues: 2-341; Coverage: 100%)
Gene names: SLG_12520, ligJ
Sequence domains: Amidohydrolase

Ligands and Environments

2 bound ligands:
Ligand ZN 4 x ZN
Ligand HJ7 4 x HJ7
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P21
Unit cell:
a: 82.689Å b: 78.253Å c: 136.216Å
α: 90° β: 89.98° γ: 90°
R-values:
R R work R free
0.206 0.202 0.266
Expression system: Escherichia coli

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