X-ray diffraction
1.65Å resolution

Crystal structure of the LigJ hydratase E284Q mutant substrate complex with (3Z)-2-keto-4-carboxy-3-hexenedioate


Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2-keto-4-carboxy-3-hexenedioate hydratase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 342 amino acids
Theoretical weight: 38.2 KDa
Source organism: Sphingobium sp. SYK-6
Expression system: Escherichia coli
  • Canonical: G2IQQ5 (Residues: 2-341; Coverage: 100%)
Gene names: SLG_12520, ligJ
Sequence domains: Amidohydrolase

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P21
Unit cell:
a: 82.689Å b: 78.253Å c: 136.216Å
α: 90° β: 89.98° γ: 90°
R R work R free
0.206 0.202 0.266
Expression system: Escherichia coli