› 6dxc

X-ray diffraction
1.54Å resolution

Crystal structure of chalcone synthase from Selaginella moellendorffii - S340C mutant

Released:
DOI: 10.2210/pdb6dxc/pdb
PDB entry 6dxc coloured by chain, front view.
PDB entry 6dxc coloured by chain, side view.
PDB entry 6dxc coloured by chain, top view.
Source organism: Selaginella moellendorffii
Primary publication:
Mechanistic basis for the evolution of chalcone synthase catalytic cysteine reactivity in land plants.
Liou G, Chiang YC, Wang Y, Weng JK
J Biol Chem 293 18601-18612 (2018)
PMID: 30291143

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112917 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chalcone synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 386 amino acids
Theoretical weight: 42.04 KDa
Source organism: Selaginella moellendorffii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D8S128 (Residues: 1-386; Coverage: 100%)
Gene name: SELMODRAFT_271225
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CSD 2 x CSD

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P21
Unit cell:
a: 55.22Å b: 66.38Å c: 103Å
α: 90° β: 91.73° γ: 90°
R-values:
R R work R free
0.143 0.142 0.172
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Flavonoid Production: Current Trends in Plant Metabolic Engineering and De Novo Microbial Production.
Tariq et al. (2023)
3 more

1 mention without citation

Mechanistic basis for the evolution of chalcone synthase catalytic cysteine reactivity in land plants.
Liou et al. (2018)