Structure analysis

Hermes transposase deletion dimer complex with (C/G) DNA

X-ray diffraction
3.2Å resolution
Source organism: Musca domestica
Assembly composition:
hetero octamer (preferred)
Entry contents: 1 distinct polypeptide molecule
3 distinct DNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero octamer
Accessible surface area: 53700 Å2
Buried surface area: 20900 Å2
Dissociation area: 1,200 Å2
Dissociation energy (ΔGdiss): 23 kcal/mol
Dissociation entropy (TΔSdiss): 18 kcal/mol
Interface energy (ΔGint): -166 kcal/mol
Symmetry number: 2

Macromolecules

Chains: A, E
Length: 517 amino acids
Theoretical weight: 59.03 KDa
Source organism: Musca domestica
Expression system: Escherichia coli
UniProt:
  • Canonical: Q25438 (Residues: 80-612; Coverage: 84%)
Pfam: hAT family C-terminal dimerisation region
InterPro:

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Name: DNA (5'-D(*GP*AP*GP*AP*AP*CP*AP*AP*CP*AP*AP*CP*AP*AP*G)-3')
Representative chains: B, F
Source organism: Musca domestica [7370]
Expression system: Not provided
Length: 15 nucleotides
Theoretical weight: 4.63 KDa

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Name: DNA (26-MER)
Representative chains: C, G
Source organism: Musca domestica [7370]
Expression system: Not provided
Length: 26 nucleotides
Theoretical weight: 7.98 KDa

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Name: DNA (5'-D(*GP*CP*GP*TP*GP*AP*C)-3')
Representative chains: D, H
Source organism: Musca domestica [7370]
Expression system: Not provided
Length: 7 nucleotides
Theoretical weight: 2.14 KDa

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