PDBe 6dwu

X-ray diffraction
3.96Å resolution

Crystal structure of complex of BBKI and Bovine Trypsin

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cationic trypsin Chains: AA, AC, AE, AG, AI, AK, AM, AO, AQ, AS, AU, BA, BC, BE, BG, BI, BK, BM, BO, BQ, BS, BU, CA, CC, CE, CG, CI, CK, CM, CO, CQ, CS, CU, DA, DC, DE, DG, DI, DK, DM, DO, DQ, DS, DU
Molecule details ›
Chains: AA, AC, AE, AG, AI, AK, AM, AO, AQ, AS, AU, BA, BC, BE, BG, BI, BK, BM, BO, BQ, BS, BU, CA, CC, CE, CG, CI, CK, CM, CO, CQ, CS, CU, DA, DC, DE, DG, DI, DK, DM, DO, DQ, DS, DU
Length: 223 amino acids
Theoretical weight: 23.32 KDa
Source organism: Bos taurus
Expression system: Bos taurus
UniProt:
  • Canonical: NEW P00760 (Residues: 24-246; Coverage: 97%)
Sequence domains: Trypsin
Kunitz-type inihibitor Chains: AB, AD, AF, AH, AJ, AL, AN, AP, AR, AT, AV, BB, BD, BF, BH, BJ, BL, BN, BP, BR, BT, BV, CB, CD, CF, CH, CJ, CL, CN, CP, CR, CT, CV, DB, DD, DF, DH, DJ, DL, DN, DP, DR, DT, DV
Molecule details ›
Chains: AB, AD, AF, AH, AJ, AL, AN, AP, AR, AT, AV, BB, BD, BF, BH, BJ, BL, BN, BP, BR, BT, BV, CB, CD, CF, CH, CJ, CL, CN, CP, CR, CT, CV, DB, DD, DF, DH, DJ, DL, DN, DP, DR, DT, DV
Length: 163 amino acids
Theoretical weight: 17.84 KDa
Source organism: Bauhinia bauhinioides
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW Q6VEQ7 (Residues: 19-181; Coverage: 93%)
Sequence domains: Trypsin and protease inhibitor

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 137.167Å b: 483.88Å c: 137.01Å
α: 90° β: 116.76° γ: 90°
R-values:
R R work R free
0.229 0.226 0.282
Expression systems:
  • Bos taurus
  • Escherichia coli