X-ray diffraction
1.2Å resolution

1.2 Angstrom Resolution Crystal Structure of Nucleoside Triphosphatase NudI from Klebsiella pneumoniae in Complex with HEPES

Entry authors: Minasov G, Shuvalova L, Pshenychnyi S, Endres M, Satchell KJF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reactions catalysed:
A nucleoside triphosphate + H(2)O = a nucleotide + diphosphate
dUTP + H(2)O = dUMP + diphosphate
dCTP + H(2)O = dCMP + diphosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Nucleoside triphosphatase NudI Chains: A, B
Molecule details ›
Chains: A, B
Length: 144 amino acids
Theoretical weight: 16.65 KDa
Source organism: Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044
Expression system: Escherichia coli BL21(DE3)
  • Canonical: A0A1W1Q5B1 (Residues: 1-141; Coverage: 100%)
Gene names: NCTC11679_01637, SAMEA3512100_01068, nudI
Sequence domains: NUDIX domain
Structure domains: Nucleoside Triphosphate Pyrophosphohydrolase

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P1
Unit cell:
a: 33.14Å b: 39.346Å c: 56.294Å
α: 77.07° β: 80.74° γ: 86.2°
R R work R free
0.155 0.153 0.187
Expression system: Escherichia coli BL21(DE3)