› 6dsp

X-ray diffraction
1.37Å resolution

LsrB from Clostridium saccharobutylicum in complex with AI-2

Released:
DOI: 10.2210/pdb6dsp/pdb
PDB entry 6dsp coloured by chain, front view.
PDB entry 6dsp coloured by chain, side view.
PDB entry 6dsp coloured by chain, top view.
Source organism: Clostridium saccharobutylicum DSM 13864
Primary publication:
Identification of novel autoinducer-2 receptors in Clostridia reveals plasticity in the binding site of the LsrB receptor family.
Torcato IM, Kasal MR, Brito PH, Miller ST, Xavier KB
J Biol Chem 294 4450-4463 (2019)
PMID: 30696769

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197559 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Periplasmic binding protein domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 347 amino acids
Theoretical weight: 37.38 KDa
Source organism: Clostridium saccharobutylicum DSM 13864
Expression system: Escherichia coli
UniProt:
  • Canonical: U5MRH9 (Residues: 24-366; Coverage: 99%)
Gene names: CLSA_c21430, lsrB
Sequence domains: Periplasmic binding protein domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand PAV 2 x PAV
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL14-1
Spacegroup: P4322
Unit cell:
a: 90.78Å b: 90.78Å c: 182.81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.159 0.177
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The role of solute binding proteins in signal transduction.
Matilla et al. (2021)
7 other articles

3 mentions without citation

Identification of novel autoinducer-2 receptors in Clostridia reveals plasticity in the binding site of the LsrB receptor family.
Torcato et al. (2019)
2 more