6do1

X-ray diffraction
2.9Å resolution

Structure of nanobody-stabilized angiotensin II type 1 receptor bound to S1I8

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only tetramer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Soluble cytochrome b562; Type-1 angiotensin II receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 425 amino acids
Theoretical weight: 48.51 KDa
Source organisms: Expression system: Homo sapiens
UniProt:
  • Canonical: P0ABE7 (Residues: 24-122; Coverage: 93%)
  • Canonical: P30556 (Residues: 2-226, 227-319; Coverage: 89%)
Gene names: AGTR1, AGTR1A, AGTR1B, AT2R1, AT2R1B, cybC
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Nanobody Nb.AT110i1 Chains: C, D, E, F
Molecule details ›
Chains: C, D, E, F
Length: 126 amino acids
Theoretical weight: 13.71 KDa
Source organism: synthetic construct
Expression system: Not provided
Angiotensin-like peptide S1I8 Chains: G, H
Molecule details ›
Chains: G, H
Length: 8 amino acids
Theoretical weight: 970 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21212
Unit cell:
a: 135.682Å b: 227.796Å c: 41.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.308 0.304 0.359
Expression systems:
  • Homo sapiens
  • Not provided