PDBe 6df9

X-ray diffraction
2.32Å resolution

Kaiso (ZBTB33) E535Q zinc finger DNA binding domain in complex with the specific Kaiso binding sequence (KBS)

Released:
Source organism: Homo sapiens
Entry authors: Nikolova EN, Stanfield RL, Dyson HJ, Wright PE

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcriptional regulator Kaiso Chain: A
Molecule details ›
Chain: A
Length: 134 amino acids
Theoretical weight: 16.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q86T24 (Residues: 471-604; Coverage: 20%)
Gene names: KAISO, ZBTB33, ZNF348
DNA (5'-D(*TP*GP*CP*TP*TP*CP*CP*TP*GP*CP*CP*AP*AP*TP*AP*AP*CP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 18 nucleotides
Theoretical weight: 5.45 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*CP*GP*TP*TP*AP*TP*TP*GP*GP*CP*AP*GP*GP*AP*AP*GP*CP*A)-3') Chain: E
Molecule details ›
Chain: E
Length: 18 nucleotides
Theoretical weight: 5.58 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: C222
Unit cell:
a: 44.452Å b: 183.142Å c: 105.683Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.172 0.218
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided