X-ray diffraction
1.63Å resolution

The structure of HcRed7, a brighter and red-shifted HcRed variant

Source organism: Heteractis crispa
Primary publication:
Monomerization of far-red fluorescent proteins.
Proc. Natl. Acad. Sci. U.S.A. (2018)
PMID: 30425172

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
homo tetramer (preferred)
homo dimer
Entry contents:
1 distinct polypeptide molecule
GFP-like non-fluorescent chromoprotein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 235 amino acids
Theoretical weight: 26.83 KDa
Source organism: Heteractis crispa
Expression system: Escherichia coli
  • Canonical: Q95W85 (Residues: 1-227; Coverage: 100%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P21
Unit cell:
a: 54.342Å b: 122.057Å c: 75.254Å
α: 90° β: 108.75° γ: 90°
R R work R free
0.178 0.178 0.214
Expression system: Escherichia coli