› 6ded

X-ray diffraction
2.2Å resolution

Crystal structure of the C-terminal ARM domain of Homo sapiens SPIN90 (SH3-protein interacting with Nck), residues 351-722

Released:
DOI: 10.2210/pdb6ded/pdb
PDB entry 6ded coloured by chain, front view.
PDB entry 6ded coloured by chain, side view.
PDB entry 6ded coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the nucleation-promoting factor SPIN90 bound to the actin filament nucleator Arp2/3 complex.
Luan Q, Liu SL, Helgeson LA, Nolen BJ
EMBO J 37 (2018)
PMID: 30322896

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192773 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NCK-interacting protein with SH3 domain Chains: A, B
Molecule details ›
Chains: A, B
Length: 373 amino acids
Theoretical weight: 42.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NZQ3 (Residues: 350-722; Coverage: 52%)
Gene names: AF3P21, NCKIPSD, SPIN90
Sequence domains: SPIN90/Ldb17, leucine-rich domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P21
Unit cell:
a: 76.365Å b: 76.17Å c: 92.257Å
α: 90° β: 93.12° γ: 90°
R-values:
R R work R free
0.216 0.215 0.239
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Nucleation, stabilization, and disassembly of branched actin networks.
Gautreau et al. (2022)
1 more

1 mention without citation

Structure of the nucleation-promoting factor SPIN90 bound to the actin filament nucleator Arp2/3 complex.
Luan et al. (2018)