Protein Data Bank in Europe

Hydrolysis of terminal, non-reducing branched (1->3)-alpha-D-galactosidic residues, producing free D-galactose

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate

Acetyl-CoA + [acyl-carrier-protein] = CoA + acetyl-[acyl-carrier-protein]

dUTP + H(2)O = dUMP + diphosphate

Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate

ATP + a protein = ADP + a phosphoprotein

UDP-N-acetyl-alpha-D-glucosamine = UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-hex-4-ulose + H(2)O

L-homoserine + NAD(P)(+) = L-aspartate 4-semialdehyde + NAD(P)H

H(2) + oxidized coenzyme F420 = reduced coenzyme F420

Chloride + O(2) = chlorite

(1a) acetyl-CoA + [acetyl-CoA C-acetyltransferase]-L-cysteine = [acetyl-CoA C-acetyltransferase]-S-acetyl-L-cysteine + CoA

ATP-dependent breakage, passage and rejoining of double-stranded DNA

Oligoglycosylglucosyl-(1<->1)-ceramide + H(2)O = ceramide + oligoglycosylglucose

Release of N-terminal proline from a peptide.

Acetyl-CoA + maltose = CoA + acetyl-maltose

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.

Carbamoyl phosphate + N(2)-acetyl-L-ornithine = phosphate + N-acetyl-L-citrulline

Myo-inositol + NAD(+) = 2,4,6/3,5-pentahydroxycyclohexanone + NADH

A phosphate monoester + H(2)O = an alcohol + phosphate

Cleavage of peptide bonds with very broad specificity.

Formamide + H(2)O = formate + NH(3)

Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.

(1a) L-glutamine + H(2)O = L-glutamate + NH(3)

4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides

ATP + H(2)O + 4 H(+)(Side 1) = ADP + phosphate + 4 H(+)(Side 2)

Chorismate = prephenate

Urea + H(2)O = CO(2) + 2 NH(3)

Xyloglucan + H(2)O = xyloglucan oligosaccharides

Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO(2) + [acyl-carrier-protein]

Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.

ATP + riboflavin = ADP + FMN

(Ribonucleotide)(n)-2',3'-cyclic phosphate + H(2)O = (ribonucleotide)(n)-2'-phosphate

Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins

N-carbamoyl-L-2-amino acid (a 2-ureido carboxylate) + H(2)O = L-2-amino acid + NH(3) + CO(2)

Protein N(6)-(dihydrolipoyl)lysine + NAD(+) = protein N(6)-(lipoyl)lysine + NADH

2 glutathione + ROOH = glutathione disulfide + H(2)O + ROH

Nitric oxide + H(2)O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H(+)

An acyl-[acyl-carrier protein] + NADP(+) = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADPH

Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil

Protein dithiol + NAD(P)(+) = protein disulfide + NAD(P)H

Hydrolysis of Xaa-|-Pro dipeptides. Also acts on aminoacyl-hydroxyproline analogs. No action on Pro-|-Pro.

N-acetyl-O-acetylneuraminate + H(2)O = N-acetylneuraminate + acetate

An acyl-[acyl-carrier protein] + NAD(+) = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH

Tetradecanoyl-CoA + an N-terminal-glycyl-[protein] = CoA + an N-terminal-N-tetradecanoylglycyl-[protein]

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu)

NTP + H(2)O = NDP + phosphate

4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O

Succinate semialdehyde + NADP(+) + H(2)O = succinate + NADPH

A (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl-carrier protein] + H(2)O

TSAVLQ-|-SGFRK-NH(2) and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.

S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA

10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide

(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH

A beta-lactam + H(2)O = a substituted beta-amino acid

5,6-dihydrouracil + H(2)O = 3-ureidopropanoate

A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid

Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.

Oleoyl-[acyl-carrier-protein] + H(2)O = [acyl-carrier-protein] + oleate

ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

RX + glutathione = HX + R-S-glutathione

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP

ATP = 3',5'-cyclic AMP + diphosphate

L-methionine + thioredoxin disulfide + H(2)O = L-methionine (S)-S-oxide + thioredoxin

D-ribose 5-phosphate = D-ribulose 5-phosphate

Similar to that of papain.

L-arginine + 2-oxoglutarate + O(2) = (3S)-3-hydroxy-L-arginine + succinate + CO(2)

Formate + NAD(+) = CO(2) + NADH

ATP + pantetheine 4'-phosphate = diphosphate + 3'-dephospho-CoA

ATP + FMN = diphosphate + FAD

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)

5'-deoxyadenosine + H(2)O = 5-deoxy-D-ribose + adenine

Reduced plastocyanin + oxidized ferredoxin + light = oxidized plastocyanin + reduced ferredoxin

Peptidylproline (omega=180) = peptidylproline (omega=0)

1D-chiro-inositol + NAD(+) = 2D-2,3,5/4,6-pentahydroxycyclohexanone + NADH

D-glyceraldehyde 3-phosphate = glycerone phosphate

L-glutamate = D-glutamate

(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide

Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans

(R)-lactate + NAD(+) = pyruvate + NADH

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate

Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacetyl-[acyl-carrier-protein] + CoA + CO(2) 

L-threonine = glycine + acetaldehyde

Methylamine + H(2)O + 2 oxidized [amicyanin] = formaldehyde + NH(3) + 2 reduced [amicyanin]

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

ATP + H(2)O = ADP + phosphate

Inosine 5'-phosphate + NAD(+) + H(2)O = xanthosine 5'-phosphate + NADH

Anthranilate + N,N-dimethyl-1,4-phenylenediamine + 2 NAD(+) = 2-(4-dimethylaminophenyl)diazenylbenzoate + 2 NADH

(1a) cyanate + HCO(3)(-) + H(+) = carbamate + CO(2)

ATP + nucleoside diphosphate = ADP + nucleoside triphosphate

Acetyl-CoA + a 2-deoxystreptamine antibiotic = CoA + N(3)-acetyl-2-deoxystreptamine antibiotic

(S)-lactate + NAD(+) = pyruvate + NADH

S-methyl-5'-thioadenosine + H(2)O = 5-(methylsulfanyl)-D-ribose + adenine

Acetyl-CoA + n malonyl-CoA + 2n NADPH = a long-chain fatty acid + (n+1) CoA + n CO(2) + 2n NADP(+)

An alpha-L-fucoside + H(2)O = L-fucose + an alcohol

(GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + undecaprenyl diphosphate

Methyl-CoM + CoB = CoM-S-S-CoB + methane

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met)

Beta-D-ribopyranose = beta-D-ribofuranose

An aliphatic amide = a nitrile + H(2)O

ATP + glycerol = ADP + sn-glycerol 3-phosphate

Malonyl-CoA + an [acyl-carrier-protein] = CoA + a malonyl-[acyl-carrier-protein]

Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.

UDP-alpha-D-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate

ATP + H(2)O + a folded polypeptide = ADP + phosphate + an unfolded polypeptide

Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose

Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe)

L-proline + NAD(P)(+) = 1-pyrroline-5-carboxylate + NAD(P)H

5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH

N-carbamoylputrescine + H(2)O = putrescine + CO(2) + NH(3)

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate

(R)-lactate + N-acetyl-D-glucosamine 6-phosphate = N-acetylmuramate 6-phosphate + H(2)O

UDP-alpha-D-glucose = UDP-alpha-D-galactose

D-ribulose 5-phosphate = D-xylulose 5-phosphate

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.

Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides

Hydrolysis of proteins in presence of ATP.

Phosphoenolpyruvate + D-erythrose 4-phosphate + H(2)O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate

D-glucosyl-N-acylsphingosine + H(2)O = D-glucose + N-acylsphingosine

Release of an N-terminal pyroglutamyl group from a polypeptide, the second amino acid generally not being Pro.

Uridine + phosphate = uracil + alpha-D-ribose 1-phosphate 

A beta-D-glucuronoside + H(2)O = D-glucuronate + an alcohol

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans

(1a) [acetyl-CoA C-acyltransferase]-S-acyl-L-cyteine + acetyl-CoA = 3-oxoacyl-CoA + [acetyl-CoA C-acyltransferase]-L-cyteine

Isochorismate + H(2)O = (2S,3S)-2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate

6-hydroxymethyl-7,8-dihydropterin diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H(2)O = ribonucleoside diphosphate + thioredoxin

Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate

2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + H(2)O = 5-amino-6-(5-phosphoribosylamino)uracil + NH(3)

3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid

L-cystathionine + H(2)O = L-homocysteine + NH(3) + pyruvate

Sucrose + (6)-beta-D-fructofuranosyl-(2->)(n) alpha-D-glucopyranoside = glucose + (6)-beta-D-fructofuranosyl-(2->)(n+1) alpha-D-glucopyranoside

Autocatalytic release of the core protein from the N-terminus of the togavirus structural polyprotein by hydrolysis of a -Trp-|-Ser- bond.

(1a) L-cystathionine = L-cysteine + 2-aminobut-2-enoate

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide

Orotidine 5'-phosphate = UMP + CO(2)

Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'.

L-erythro-3,5-diaminohexanoate + H(2)O + NAD(+) = (S)-5-amino-3-oxohexanoate + NH(3) + NADH

L-glutamate 5-semialdehyde + NAD(+) + H(2)O = L-glutamate + NADH

5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate

A long-chain aldehyde + O(2) + 2 NADPH = an alkane + formate + H(2)O + 2 NADP(+)

(1a) L-glutamine + H(2)O = L-glutamate + NH(4)(+)

Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.

Isocitrate = succinate + glyoxylate

3-deoxy-D-manno-octulosonate 8-phosphate + H(2)O = 3-deoxy-D-manno-octulosonate + phosphate

S-adenosyl 3-(methylthio)propylamine + putrescine = 5'-S-methyl-5'-thioadenosine + spermidine

D-xylopyranose = D-xylulose

(R)-mandelonitrile = cyanide + benzaldehyde

5-amino-6-(5-phospho-D-ribitylamino)uracil + NADP(+) = 5-amino-6-(5-phospho-D-ribosylamino)uracil + NADPH

5,10-methylenetetrahydrofolate + glycine + H(2)O = tetrahydrofolate + L-serine

2 phenolic donor + H(2)O(2) = 2 phenoxyl radical of the donor + 2 H(2)O

Alpha-maltose 1-phosphate + ((1->4)-alpha-D-glucosyl)(n) = phosphate + ((1->4)-alpha-D-glucosyl)(n+2)

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose

H(2)CO(3) = CO(2) + H(2)O

S-(hydroxymethyl)glutathione + NAD(P)(+) = S-formylglutathione + NAD(P)H

O-phospho-L-homoserine + H(2)O = L-threonine + phosphate

Preferential cleavage of bonds with hydrophobic residues in P1'.

S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA 

L-proline + a quinone = (S)-1-pyrroline-5-carboxylate + a quinol

L-lysine + O(2) = 5-aminopentanamide + CO(2) + H(2)O

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate

Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides

Protein L-glutamine + H(2)O = protein L-glutamate + NH(3)

Maltose = alpha,alpha-trehalose

10-formyltetrahydrofolate + H(2)O = formate + tetrahydrofolate

ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec)

L-ornithine = L-proline + NH(3)

Betaine aldehyde + NAD(+) + H(2)O = betaine + NADH

An aldehyde + NAD(+) + H(2)O = a carboxylate + NADH

Penicillin + H(2)O = a carboxylate + 6-aminopenicillanate

O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate

UDP-N-acetyl-alpha-D-glucosamine + (1,4-(N-acetyl-beta-D-glucosaminyl))(n) = UDP + (1,4-(N-acetyl-beta-D-glucosaminyl))(n+1)

Thioredoxin + ROOH = thioredoxin disulfide + H(2)O + ROH

2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O

Random hydrolysis of (1->6)-alpha-D-mannosidic linkages in unbranched (1->6)-mannans

ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr)

A protein-L-glutamine + a protein-L-lysine = a protein with an N(6)-(gamma-glutamyl)-L-lysine cross-link + NH(3)

A primary alcohol + NAD(+) = an aldehyde + NADH

A pyrimidine nucleoside + H(2)O = D-ribose + a pyrimidine base

2 3-phospho-D-glycerate + 2 H(+) = D-ribulose 1,5-bisphosphate + CO(2) + H(2)O

n ATP + n H(2)O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers

S-adenosyl-L-methionine + ethanolamine phosphate = S-adenosyl-L-homocysteine + N-methylethanolamine phosphate

3-dehydroquinate = 3-dehydroshikimate + H(2)O

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro)

IMP + H(2)O = inosine + phosphate

10-formyltetrahydrofolate + NADP(+) + H(2)O = tetrahydrofolate + CO(2) + NADPH

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp)

4 sulfur + 4 H(2)O + O(2) = 2 H(2)S + 2 HSO(3)(-) + 2 H(+)

Triacylglycerol + H(2)O = diacylglycerol + a carboxylate

4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate

(S)-mandelate = (R)-mandelate

Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate

(S)-3-hydroxybutanoyl-CoA + NADP(+) = 3-acetoacetyl-CoA + NADPH

Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate

Acts on substrates that are at least partially unfolded. The cleavage site P1 residue is normally between a pair of hydrophobic residues, such as Val-|-Val

8 reduced ferredoxin + 8 H(+) + N(2) + 16 ATP + 16 H(2)O = 8 oxidized ferredoxin + H(2) + 2 NH(3) + 16 ADP + 16 phosphate

Cyclic pyranopterin phosphate + 2 [molybdopterin-synthase sulfur-carrier protein]-Gly-NH-CH(2)-C(O)SH + H(2)O = molybdopterin + 2 [molybdopterin-synthase sulfur-carrier protein]

1-haloalkane + H(2)O = a primary alcohol + halide

(R)-mevalonate + CoA + 2 NAD(+) = 3-hydroxy-3-methylglutaryl-CoA + 2 NADH

(1a) a nucleoside 5'-triphosphate + H(2)O = a nucleoside 5'-diphosphate + phosphate

Beta-nicotinate D-ribonucleotide + 5,6-dimethylbenzimidazole = nicotinate + alpha-ribazole 5'-phosphate

(+)-muconolactone = cis,cis-muconate

Endopeptidase with a preference for cleavage when the P1 position is occupied by Glu-|- and the P1' position is occupied by Gly-|-

((1->4)-alpha-D-glucosyl)(n) + phosphate = ((1->4)-alpha-D-glucosyl)(n-1) + alpha-D-glucose 1-phosphate

5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO(2)

Urate + O(2) + H(2)O = 5-hydroxyisourate + H(2)O(2)

Produces alpha-D-fructofuranose beta-D-fructofuranose 1,2':2,1'-dianhydride (DFA I) by successively eliminating the diminishing (2->1)-beta-D-fructan (inulin) chain from the terminal D-fructosyl-D-fructosyl disaccharide.

D-sorbitol 6-phosphate + NAD(+) = D-fructose 6-phosphate + NADH

ATP + L-glutamate + NH(3) = ADP + phosphate + L-glutamine

6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H(2)O = D-glucose + D-glucose 6-phosphate

dTDP-4-dehydro-6-deoxy-alpha-D-glucose = dTDP-4-dehydro-beta-L-rhamnose

GDP-alpha-D-mannose = GDP-4-dehydro-alpha-D-rhamnose + H(2)O

D-glucarate = 5-dehydro-4-deoxy-D-glucarate + H(2)O

(1a) dTDP-beta-L-evernosamine + NADPH + O(2) = dTDP-N-hydroxy-beta-L-evernosamine + NADP(+) + H(2)O

Myo-inositol phosphate + H(2)O = myo-inositol + phosphate

S-(5-deoxy-D-ribos-5-yl)-L-homocysteine = L-homocysteine + (4S)-4,5-dihydroxypentan-2,3-dione

2 H(2)O(2) = O(2) + 2 H(2)O

Taurine + 2-oxoglutarate + O(2) = sulfite + aminoacetaldehyde + succinate + CO(2)

(R)-3-hydroxybutanoate + NAD(+) = acetoacetate + NADH

2 superoxide + 2 H(+) = O(2) + H(2)O(2)

ADP-alpha-D-glucose + D-glucose 6-phosphate = ADP + alpha,alpha-trehalose 6-phosphate

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate

D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + NADPH

D-glucose + NAD(P)(+) = D-glucono-1,5-lactone + NAD(P)H

UDP-N-acetyl-alpha-D-mannosamine + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol = UDP + N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol

S-adenosyl-L-methionine + uracil(1498) in 16S rRNA = S-adenosyl-L-homocysteine + N(3)-methyluracil(1498) in 16S rRNA 

L-histidinol + H(2)O + 2 NAD(+) = L-histidine + 2 NADH

SsDNA + n NTP = ssDNA/pppN(pN)(n-1) hybrid + (n-1) diphosphate

ATP + thiamine = AMP + thiamine diphosphate

A (3R)-3-hydroxyacyl-[acyl-carrier-protein] + a UDP-3-O-((3R)-hydroxyacyl)-alpha-D-glucosamine = a UDP-2-N,3-O-bis((3R)-3-hydroxyacyl)-alpha-D-glucosamine + a holo-[acyl-carrier-protein]

5-hydroxyisourate + H(2)O = 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate

Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins

Succinate + a quinone = fumarate + a quinol

An aldehyde + NAD(P)(+) + H(2)O = a carboxylate + NAD(P)H

D-glucono-1,5-lactone + H(2)O = D-gluconate

Phenylacetaldehyde + NAD(+) + H(2)O = phenylacetate + NADH

7,8-dihydroneopterin = 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde

O(4)-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate

2 glutathione + NADP(+) = glutathione disulfide + NADPH

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O

(S)-malate + NAD(+) = oxaloacetate + NADH

5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate

N(6)-prenyladenine + acceptor + H(2)O = adenine + 3-methylbut-2-enal + reduced acceptor

2-C-methyl-D-erythritol 4-phosphate + NADP(+) = 1-deoxy-D-xylulose 5-phosphate + NADPH

ATP + propanoyl-CoA + HCO(3)(-) = ADP + phosphate + (S)-methylmalonyl-CoA

Phosphatidylinositol 3,4,5-trisphosphate + H(2)O = phosphatidylinositol 4,5-bisphosphate + phosphate

NADPH + n oxidized hemoprotein = NADP(+) + n reduced hemoprotein

Quercetin + O(2) = 2-(3,4-dihydroxybenzoyloxy)-4,6-dihydroxybenzoate + CO + H(+)

RH + [reduced NADPH--hemoprotein reductase] + O(2) = ROH + [oxidized NADPH--hemoprotein reductase] + H(2)O

Diphosphate + H(2)O = 2 phosphate

L-lysine + NADPH + O(2) = N(6)-hydroxy-L-lysine + NADP(+) + H(2)O

N-substituted aminoacyl-tRNA + H(2)O = N-substituted amino acid + tRNA

Acetyl-CoA + L-serine = CoA + O-acetyl-L-serine

L-serine + L-homocysteine = L-cystathionine + H(2)O

ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO(3)(-) = ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole

Pyridoxamine 5'-phosphate + H(2)O + O(2) = pyridoxal 5'-phosphate + NH(3) + H(2)O(2)

Prephenate = phenylpyruvate + H(2)O + CO(2)

L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate

ATP + pyruvate = ADP + phosphoenolpyruvate

(1a) ATP + [DNA ligase]-L-lysine = [DNA ligase]-N(6)-(5'-adenylyl)-L-lysine + diphosphate

ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA

dTTP + alpha-D-glucose 1-phosphate = diphosphate + dTDP-alpha-D-glucose

Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate

3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholan-24-oylglycine + H(2)O = 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholanate + glycine

A (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a (Z)-3-oxooctadec-11-enoyl-[acyl-carrier protein] + CO(2) + an [acyl-carrier protein]

Acetyl-CoA + L-2,4-diaminobutanoate = CoA + N(4)-acetyl-L-2,4-diaminobutanoate

Hydrolyzes glutaminyl bonds, and activity is further restricted by preferences for the amino acids in P6 - P1' that vary with the species of potyvirus, e.g. Glu-Xaa-Xaa-Tyr-Xaa-Gln-|-(Ser or Gly) for the enzyme from tobacco etch virus. The natural substrate is the viral polyprotein, but other proteins and oligopeptides containing the appropriate consensus sequence are also cleaved.

Hydrolyzes a Gly-|-Gly bond at its own C-terminus, commonly in the sequence -Tyr-Xaa-Val-Gly-|-Gly, in the processing of the potyviral polyprotein.

2 bilirubin + O(2) = 2 biliverdin + 2 H(2)O

Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose

D-fructose 1,6-bisphosphate + H(2)O = D-fructose 6-phosphate + phosphate

N,N-dimethylformamide + H(2)O = dimethylamine + formate

2-deoxy-D-ribose 5-phosphate = D-glyceraldehyde 3-phosphate + acetaldehyde

ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate

Prenyl phosphate + FMNH(2) = prenylated FMNH(2) + phosphate

S-formylglutathione + H(2)O = glutathione + formate

Acetyl-CoA + H(2)O + oxaloacetate = citrate + CoA

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine

ATP + 6-hydroxymethyl-7,8-dihydropterin = AMP + 6-hydroxymethyl-7,8-dihydropterin diphosphate

ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp)

Oxalate + O(2) + 2 H(+) = 2 CO(2) + H(2)O(2)

[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate

Pyruvate + L-aspartate-4-semialdehyde = (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate + H(2)O

Limited hydrolysis of proteins of the neuroexocytosis apparatus, synaptobrevin (also known as neuronal vesicle-associated membrane protein, VAMP), synaptosome-associated protein of 25 kDa (SNAP25) or syntaxin. No detected action on small molecule substrates.

A C(21)-steroid + [reduced NADPH--hemoprotein reductase] + O(2) = a 17-alpha-hydroxy-C(21)-steroid + [oxidized NADPH--hemoprotein reductase] + H(2)O

17-alpha-hydroxypregnenolone + [reduced NADPH--hemoprotein reductase] + O(2) = 3-beta-hydroxyandrost-5-en-17-one + acetate + [oxidized NADPH--hemoprotein reductase] + H(2)O

Quinol + 2 ferricytochrome c(Side 2) = quinone + 2 ferrocytochrome c(Side 2) + 2 H(+)(Side 2)