› 6d94

X-ray diffraction
1.9Å resolution

Crystal structure of PPAR gamma in complex with Mediator of RNA polymerase II transcription subunit 1

Released:
DOI: 10.2210/pdb6d94/pdb
PDB entry 6d94 coloured by chain, front view.
PDB entry 6d94 coloured by chain, side view.
PDB entry 6d94 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A structural mechanism of nuclear receptor biased agonism.
Nemetchek MD, Chrisman IM, Rayl ML, Voss AH, Hughes TS
Proc Natl Acad Sci U S A 119 e2215333119 (2022)
PMID: 36469765

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153374 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Peroxisome proliferator-activated receptor gamma Chain: A
Molecule details ›
Chain: A
Length: 297 amino acids
Theoretical weight: 34.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P37231 (Residues: 231-505; Coverage: 55%)
Gene names: NR1C3, PPARG
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
Mediator of RNA polymerase II transcription subunit 1 Chain: B
Molecule details ›
Chain: B
Length: 24 amino acids
Theoretical weight: 2.62 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q15648 (Residues: 632-655; Coverage: 2%)
Gene names: ARC205, CRSP1, CRSP200, DRIP205, DRIP230, MED1, PBP, PPARBP, PPARGBP, RB18A, TRAP220, TRIP2

Ligands and Environments

1 bound ligand:
Ligand EDK 1 x EDK
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: C2221
Unit cell:
a: 55.437Å b: 87.623Å c: 121.561Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.199 0.258
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

International Union of Basic and Clinical Pharmacology CXIII: Nuclear Receptor Superfamily-Update 2023.
Burris et al. (2023)
1 other article

1 mention without citation

A structural mechanism of nuclear receptor biased agonism.
Nemetchek et al. (2022)