PDBe 6d6v

Electron Microscopy
4.8Å resolution

CryoEM structure of Tetrahymena telomerase with telomeric DNA at 4.8 Angstrom resolution

Released:
Source organism: Tetrahymena thermophila
Primary publication:
Structure of Telomerase with Telomeric DNA.
Cell 173 1179-1190.e13 (2018)
PMID: 29775593
Related structures: EMD-7821

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
6 distinct polypeptide molecules
1 distinct DNA molecule
1 distinct RNA molecule
Macromolecules (8 distinct):
Telomerase reverse transcriptase Chain: A
Molecule details ›
Chain: A
Length: 1117 amino acids
Theoretical weight: 133.49 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: O77448 (Residues: 1-1117; Coverage: 100%)
Gene name: TERT
Sequence domains:
Telomerase-associated protein 82 Chain: D
Molecule details ›
Chain: D
Length: 701 amino acids
Theoretical weight: 82.04 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: D2CVN6 (Residues: 1-701; Coverage: 100%)
Gene name: TAP82
Sequence domains:
Telomerase holoenzyme TEB heterotrimer Teb3 subunit Chain: F
Molecule details ›
Chain: F
Length: 121 amino acids
Theoretical weight: 14.01 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: A0A0U8UFF4 (Residues: 1-121; Coverage: 100%)
Sequence domains: Replication factor A protein 3
Telomerase holoenzyme Teb2 subunit Chain: E
Molecule details ›
Chain: E
Length: 269 amino acids
Theoretical weight: 30.99 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: A0A0U8TRG9 (Residues: 1-269; Coverage: 100%)
Telomerase-associated protein 50 Chain: G
Molecule details ›
Chain: G
Length: 157 amino acids
Theoretical weight: 13.38 KDa
Source organism: Tetrahymena thermophila
Telomerase associated protein p65 Chain: H
Molecule details ›
Chain: H
Length: 542 amino acids
Theoretical weight: 64.21 KDa
Source organism: Tetrahymena thermophila
UniProt:
  • Canonical: Q6JXI6 (Residues: 1-542; Coverage: 100%)
Gene name: TAP65
Sequence domains:
DNA (5'-D(P*GP*TP*TP*GP*GP*GP*GP*TP*TP*GP*GP*GP*GP*TP*TP*GP*GP*GP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 19 nucleotides
Theoretical weight: 6.06 KDa
RNA (159-MER) Chain: B
Molecule details ›
Chain: B
Length: 159 nucleotides
Theoretical weight: 50.75 KDa
Sequence domains: Ciliate telomerase RNA

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.8Å
Relevant EMDB volumes: EMD-7821