6d6u

Electron Microscopy
3.8Å resolution

Human GABA-A receptor alpha1-beta2-gamma2 subtype in complex with GABA and flumazenil, conformation A

Released:
Source organisms:
Primary publication:
Structure of a human synaptic GABAA receptor.
OpenAccess logo Nature (2018)
PMID: 29950725
Related structures: EMD-7817

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (8 distinct):
Gamma-aminobutyric acid receptor subunit beta-2 Chains: A, C
Molecule details ›
Chains: A, C
Length: 341 amino acids
Theoretical weight: 39.52 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P47870 (Residues: 25-331, 486-512; Coverage: 68%)
Gene name: GABRB2
Sequence domains:
Structure domains: Neurotransmitter-gated ion-channel ligand-binding domain
Gamma-aminobutyric acid receptor subunit alpha-1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 358 amino acids
Theoretical weight: 41.06 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P14867 (Residues: 28-339, 418-456; Coverage: 82%)
Gene name: GABRA1
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains:
Gamma-aminobutyric acid receptor subunit gamma-2 Chain: E
Molecule details ›
Chain: E
Length: 394 amino acids
Theoretical weight: 45.35 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P18507 (Residues: 40-361, 439-467; Coverage: 82%)
Gene name: GABRG2
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains: Neurotransmitter-gated ion-channel ligand-binding domain
Kappa Fab Light Chain Chains: I, L
Molecule details ›
Chains: I, L
Length: 213 amino acids
Theoretical weight: 23.51 KDa
Source organism: Mus musculus
IgG2b Fab Heavy Chain Chains: J, K
Molecule details ›
Chains: J, K
Length: 454 amino acids
Theoretical weight: 49.81 KDa
Source organism: Mus musculus

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-7817
Expression system: Homo sapiens