› 6d6l

X-ray diffraction
1.63Å resolution

The structure of ligand binding domain of LasR in complex with TP-1 homolog, compound 14

Released:
DOI: 10.2210/pdb6d6l/pdb
PDB 6d6l coloured by chain and viewed from the front.
PDB 6d6l coloured by chain and viewed from the side.
PDB 6d6l coloured by chain and viewed from the top.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Structural and Biochemical Studies of Non-native Agonists of the LasR Quorum-Sensing Receptor Reveal an L3 Loop "Out" Conformation for LasR.
O'Reilly MC, Dong SH, Rossi FM, Karlen KM, Kumar RS, Nair SK, Blackwell HE
Cell Chem Biol 25 1128-1139.e3 (2018)
PMID: 30033130

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcriptional activator protein LasR Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 170 amino acids
Theoretical weight: 19.01 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: P25084 (Residues: 1-170; Coverage: 71%)
Gene names: PA1430, lasR
Sequence domains: Autoinducer binding domain
Structure domains: Transcription factor LuxR-like, autoinducer-binding domain

Ligands and Environments

1 bound ligand:
Ligand FY4 4 x FY4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 56.12Å b: 84.55Å c: 76.99Å
α: 90° β: 101.09° γ: 90°
R-values:
R R work R free
0.221 0.22 0.24
Expression system: Escherichia coli

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Citations

15 citation in other articles

An Autoinducer Analogue Reveals an Alternative Mode of Ligand Binding for the LasR Quorum-Sensing Receptor.
Paczkowski et al. (2019)
14 more