› 6d3h

X-ray diffraction
2.03Å resolution

Function and Biology Details

Reaction catalysed: 
(R)-(2,4-dichlorophenoxy)propanoate + 2-oxoglutarate + O(2) = 2,4-dichlorophenol + pyruvate + succinate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185214 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
(R)-phenoxypropionate/alpha-ketoglutarate-dioxygenase Chains: A, B, I, M
Molecule details ›
Chains: A, B, I, M
Length: 295 amino acids
Theoretical weight: 33.45 KDa
Source organism: Sphingobium herbicidovorans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8KSC8 (Residues: 1-295; Coverage: 100%)
Gene name: rdpA
Sequence domains: Taurine catabolism dioxygenase TauD, TfdA family
Structure domains: Clavaminate synthase-like

Ligands and Environments

4 bound ligands:
Ligand FTV 4 x FTV
Ligand CO 4 x CO
Ligand AKG 4 x AKG
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P64
Unit cell:
a: 138.161Å b: 138.161Å c: 148.518Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.172 0.17 0.211
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Synergistic mutations of two rapeseed AHAS genes confer high resistance to sulfonylurea herbicides for weed control.
Guo et al. (2020)
1 more