› 6d2w

X-ray diffraction
2.1Å resolution

Crystal structure of Prevotella bryantii endo-beta-mannanase/endo-beta-glucanase PbGH26A-GH5A

Released:
DOI: 10.2210/pdb6d2w/pdb
PDB entry 6d2w coloured by chain, front view.
PDB entry 6d2w coloured by chain, side view.
PDB entry 6d2w coloured by chain, top view.
Source organism: Prevotella bryantii B14
Entry authors: Stogios PJ, Skarina T, McGregor N, Nocek B, Di Leo R, Brumer H, Savchenko A

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112902 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
B-1,4-endoglucanase Chains: A, B
Molecule details ›
Chains: A, B
Length: 776 amino acids
Theoretical weight: 87 KDa
Source organism: Prevotella bryantii B14
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D8DY19 (Residues: 1-776; Coverage: 100%)
Gene name: PBR_0368
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand GOL 3 x GOL
Ligand EPE 3 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 54.484Å b: 96.753Å c: 138.089Å
α: 90° β: 99.27° γ: 90°
R-values:
R R work R free
0.147 0.146 0.193
Expression system: Escherichia coli BL21(DE3)

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