6d24

X-ray diffraction
3.35Å resolution

Trypanosoma cruzi Glucose-6-P Dehydrogenase in complex with G6P

Released:
Source organism: Trypanosoma cruzi
Entry authors: Botti H, Ortiz C, Comini MA, Larrieux N, Buschiazzo A

Function and Biology Details

Reaction catalysed:
D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Glucose-6-phosphate 1-dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 541 amino acids
Theoretical weight: 60.95 KDa
Source organism: Trypanosoma cruzi
Expression system: Escherichia coli
UniProt:
  • Canonical: Q1WBU6 (Residues: 38-555; Coverage: 93%)
Gene names: C3747_142g63, G6PDH1
Sequence domains:
Structure domains:
Glucose-6-phosphate 1-dehydrogenase Chains: C, D
Molecule details ›
Chains: C, D
Length: 541 amino acids
Theoretical weight: 60.97 KDa
Source organism: Trypanosoma cruzi
Expression system: Escherichia coli
UniProt:
  • Canonical: Q1WBU6 (Residues: 38-555; Coverage: 93%)
Gene names: C3747_142g63, G6PDH1
Sequence domains:
Structure domains:

Ligands and Environments

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 96.79Å b: 133.03Å c: 107.75Å
α: 90° β: 100.27° γ: 90°
R-values:
R R work R free
0.206 0.205 0.254
Expression system: Escherichia coli