› 6d14

X-ray diffraction
2.5Å resolution

Zebrafish TRAP1 bound to AMPPNP and calcium in the asymmetric closed state

Released:
DOI: 10.2210/pdb6d14/pdb
PDB entry 6d14 coloured by chain, front view.
PDB entry 6d14 coloured by chain, side view.
PDB entry 6d14 coloured by chain, top view.
Source organism: Danio rerio
Primary publication:
Calcium binding to a remote site can replace magnesium as cofactor for mitochondrial Hsp90 (TRAP1) ATPase activity.
Elnatan D, Agard DA
J Biol Chem 293 13717-13724 (2018)
PMID: 29991590

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108806 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histidine kinase/HSP90-like ATPase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 635 amino acids
Theoretical weight: 72.64 KDa
Source organism: Danio rerio
Expression system: Escherichia coli
UniProt:
  • Canonical: A8WFV1 (Residues: 85-719; Coverage: 88%)
Gene names: fc85a11, trap1, wu:fc85a11
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ANP 2 x ANP
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2
Unit cell:
a: 176.252Å b: 95.751Å c: 124.546Å
α: 90° β: 134.66° γ: 90°
R-values:
R R work R free
0.231 0.23 0.255
Expression system: Escherichia coli

Quick links

Citations

8 review citations

The HSP90 Family: Structure, Regulation, Function, and Implications in Health and Disease.
Hoter et al. (2018)
7 more

4 mentions without citation

Dynamically Shaping Chaperones. Allosteric Modulators of HSP90 Family as Regulatory Tools of Cell Metabolism in Neoplastic Progression.
Sanchez-Martin et al. (2020)
3 more