› 6d0r

X-ray diffraction
2.86Å resolution

Structure of a DNA retention-prone PCNA variant

Released:
DOI: 10.2210/pdb6d0r/pdb
PDB entry 6d0r coloured by chain, front view.
PDB entry 6d0r coloured by chain, side view.
PDB entry 6d0r coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Effective mismatch repair depends on timely control of PCNA retention on DNA by the Elg1 complex.
Paul Solomon Devakumar LJ, Gaubitz C, Lundblad V, Kelch BA, Kubota T
Nucleic Acids Res 47 6826-6841 (2019)
PMID: 31114918

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147511 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proliferating cell nuclear antigen Chain: A
Molecule details ›
Chain: A
Length: 264 amino acids
Theoretical weight: 29.54 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P15873 (Residues: 1-258; Coverage: 100%)
Gene names: POL30, YBR0811, YBR088C
Sequence domains:
Structure domains: Proliferating Cell Nuclear Antigen

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P213
Unit cell:
a: 121.716Å b: 121.716Å c: 121.716Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.244 0.242 0.276
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

DNA Mismatch Repair and its Role in Huntington's Disease.
Iyer et al. (2021)
2 more

2 mentions without citation

Effective mismatch repair depends on timely control of PCNA retention on DNA by the Elg1 complex.
Paul Solomon Devakumar et al. (2019)
1 more