› 6d0n

X-ray diffraction
3.12Å resolution

Crystal structure of a CLC-type fluoride/proton antiporter, V319G mutant

Released:
DOI: 10.2210/pdb6d0n/pdb
PDB entry 6d0n coloured by chain, front view.
PDB entry 6d0n coloured by chain, side view.
PDB entry 6d0n coloured by chain, top view.
Source organisms:
Primary publication:
A CLC-type F-/H+ antiporter in ion-swapped conformations.
Last NB, Stockbridge RB, Wilson AE, Shane T, Kolmakova-Partensky L, Koide A, Koide S, Miller C
Nat Struct Mol Biol 25 601-606 (2018)
PMID: 29941917

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-241460 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
CLC-type fluoride/proton antiporter Chains: A, B
Molecule details ›
Chains: A, B
Length: 421 amino acids
Theoretical weight: 45.63 KDa
Source organism: Enterococcus casseliflavus EC10
Expression system: Escherichia coli BL21(DE3)
Monobody Chains: C, D
Molecule details ›
Chains: C, D
Length: 93 amino acids
Theoretical weight: 9.89 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

3 bound ligands:
Ligand DMU 6 x DMU
Ligand F 1 x F
Ligand MHA 1 x MHA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 118.106Å b: 124.843Å c: 135.482Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.249 0.248 0.27
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Monobodies as enabling tools for structural and mechanistic biology.
Hantschel et al. (2020)
2 more

1 mention without citation

A CLC-type F-/H+ antiporter in ion-swapped conformations.
Last et al. (2018)