X-ray diffraction
1.7Å resolution

Crystal structure of Arabidopsis thaliana phosphoserine aminotransferase isoform 1 (AtPSAT1) in complex with PLP-phosphoserine geminal diamine intermediate


Function and Biology Details

Reaction catalysed:
4-phosphonooxy-L-threonine + 2-oxoglutarate = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Phosphoserine aminotransferase 1, chloroplastic Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 362 amino acids
Theoretical weight: 40.28 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q96255 (Residues: 72-430; Coverage: 84%)
Gene names: At4g35630, F8D20.140, PSAT1
Sequence domains: Aminotransferase class-V
Structure domains:

Ligands and Environments

Cofactor: Ligand PLP 4 x PLP
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 189.668Å b: 53.288Å c: 137.921Å
α: 90° β: 91.91° γ: 90°
R R work R free
0.181 0.181 0.209
Expression system: Escherichia coli BL21(DE3)