› 6czx

X-ray diffraction
1.57Å resolution

Crystal structure of Arabidopsis thaliana phosphoserine aminotransferase isoform 1 (AtPSAT1) in complex with PLP internal aldimine

Released:
DOI: 10.2210/pdb6czx/pdb
PDB entry 6czx coloured by chain, front view.
PDB entry 6czx coloured by chain, side view.
PDB entry 6czx coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural Analysis of Phosphoserine Aminotransferase (Isoform 1) From Arabidopsis thaliana- the Enzyme Involved in the Phosphorylated Pathway of Serine Biosynthesis.
Sekula B, Ruszkowski M, Dauter Z
Front Plant Sci 9 876 (2018)
PMID: 30034403

Function and Biology Details

Reaction catalysed: 
4-phosphonooxy-L-threonine + 2-oxoglutarate = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188295 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoserine aminotransferase 1, chloroplastic Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 362 amino acids
Theoretical weight: 40.5 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96255 (Residues: 72-430; Coverage: 84%)
Gene names: At4g35630, F8D20.140, PSAT1
Sequence domains: Aminotransferase class-V
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 12 x SO4
Ligand PEG 3 x PEG
Ligand MRD 2 x MRD
1 modified residue:
Ligand LLP 4 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 84.221Å b: 105.929Å c: 186.886Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.157 0.175
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Structural Basis for Allostery in PLP-dependent Enzymes.
Tran et al. (2022)
7 other articles

3 mentions without citation

Insight into de-regulation of amino acid feedback inhibition: a focus on structure analysis method.
Naz et al. (2023)
2 more