› 6cyz

X-ray diffraction
2.04Å resolution

Mycobacterial homoserine kinase ThrB in complex with AMPPNP

Released:
DOI: 10.2210/pdb6cyz/pdb
PDB entry 6cyz coloured by chain, front view.
PDB entry 6cyz coloured by chain, side view.
PDB entry 6cyz coloured by chain, top view.
Source organism: Mycobacteroides abscessus ATCC 19977
Entry authors: Li J, Korotkov KV

Function and Biology Details

Reaction catalysed: 
ATP + L-homoserine = ADP + O-phospho-L-homoserine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109373 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Homoserine kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 308 amino acids
Theoretical weight: 31.07 KDa
Source organism: Mycobacteroides abscessus ATCC 19977
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B1MLU6 (Residues: 1-306; Coverage: 100%)
Gene names: MAB_1437, thrB
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ANP 2 x ANP
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P21
Unit cell:
a: 57.35Å b: 62.7Å c: 84.63Å
α: 90° β: 104.4° γ: 90°
R-values:
R R work R free
0.199 0.197 0.235
Expression system: Escherichia coli BL21(DE3)

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