› 6cyu

X-ray diffraction
1.82Å resolution

Crystal structure of CTX-M-14 S70G/N106S/D240G beta-lactamase in complex with hydrolyzed cefotaxime

Released:
DOI: 10.2210/pdb6cyu/pdb
PDB entry 6cyu coloured by chain, front view.
PDB entry 6cyu coloured by chain, side view.
PDB entry 6cyu coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Synergistic effects of functionally distinct substitutions in β-lactamase variants shed light on the evolution of bacterial drug resistance.
Patel MP, Hu L, Brown CA, Sun Z, Adamski CJ, Stojanoski V, Sankaran B, Prasad BVV, Palzkill T
J Biol Chem 293 17971-17984 (2018)
PMID: 30275013

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192212 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chain: A
Molecule details ›
Chain: A
Length: 263 amino acids
Theoretical weight: 27.89 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9L5C8 (Residues: 29-291; Coverage: 100%)
Gene names: RCS51_P0174, bla CTX-M-9, blaCTX-M-9, blaCTX-M-9a, blaCTX-M-9b
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand CE4 1 x CE4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P3221
Unit cell:
a: 41.399Å b: 41.399Å c: 230.61Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.16 0.157 0.2
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Molecular Mechanisms, Epidemiology, and Clinical Importance of β-Lactam Resistance in Enterobacteriaceae.
De Angelis et al. (2020)
12 other articles

1 mention without citation

Synergistic effects of functionally distinct substitutions in β-lactamase variants shed light on the evolution of bacterial drug resistance.
Patel et al. (2018)