› 6cw3

X-ray diffraction
1.98Å resolution

Crystal structure of a yeast SAGA transcriptional coactivator Ada2/Gcn5 HAT subcomplex, crystal form 2

Released:
DOI: 10.2210/pdb6cw3/pdb
PDB entry 6cw3 coloured by chain, front view.
PDB entry 6cw3 coloured by chain, side view.
PDB entry 6cw3 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for activation of SAGA histone acetyltransferase Gcn5 by partner subunit Ada2.
Sun J, Paduch M, Kim SA, Kramer RM, Barrios AF, Lu V, Luke J, Usatyuk S, Kossiakoff AA, Tan S
Proc Natl Acad Sci U S A 115 10010-10015 (2018)
PMID: 30224453

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-211895 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
antibody heavy chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 234 amino acids
Theoretical weight: 25.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
antibody light chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 215 amino acids
Theoretical weight: 23.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Histone acetyltransferase GCN5 Chains: F, H
Molecule details ›
Chains: F, H
Length: 254 amino acids
Theoretical weight: 29.36 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q03330 (Residues: 67-317; Coverage: 57%)
Gene names: ADA4, GCN5, SWI9, YGR252W
Sequence domains: Acetyltransferase (GNAT) family
Transcriptional adapter 2 Chains: E, G
Molecule details ›
Chains: E, G
Length: 122 amino acids
Theoretical weight: 13.86 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02336 (Residues: 2-120; Coverage: 27%)
Gene names: ADA2, D9461.33, YDR448W
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 101.657Å b: 104.061Å c: 166.335Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.202 0.227
Expression systems:
  • Escherichia coli
  • Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

The SAGA chromatin-modifying complex: the sum of its parts is greater than the whole.
Soffers et al. (2020)
6 more

1 mention without citation

Structural basis for activation of SAGA histone acetyltransferase Gcn5 by partner subunit Ada2.
Sun et al. (2018)