› 6cuj

X-ray diffraction
1.8Å resolution

Crystal structure of the C-terminal domain of neisserial heparin binding antigen (NHBA)

Released:
DOI: 10.2210/pdb6cuj/pdb
PDB entry 6cuj coloured by chain, front view.
PDB entry 6cuj coloured by chain, side view.
PDB entry 6cuj coloured by chain, top view.
Source organism: Neisseria meningitidis
Primary publication:
Structures of NHBA elucidate a broadly conserved epitope identified by a vaccine induced antibody.
Maritan M, Veggi D, Cozzi R, Dello Iacono L, Bartolini E, Lo Surdo P, Maruggi G, Spraggon G, Bottomley MJ, Malito E
PLoS One 13 e0201922 (2018)
PMID: 30133484

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191663 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
C2 fragment Chains: A, B
Molecule details ›
Chains: A, B
Length: 161 amino acids
Theoretical weight: 17.38 KDa
Source organism: Neisseria meningitidis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9JPP1 (Residues: 275-427; Coverage: 37%)
Gene names: gna2132, nhba
Sequence domains: C-lobe and N-lobe beta barrels of Tf-binding protein B

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P41
Unit cell:
a: 77.03Å b: 77.03Å c: 57.53Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.191 0.238
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

The Development of a Vaccine Against Meningococcus B Using Reverse Vaccinology.
Masignani et al. (2019)
4 other articles

3 mentions without citation

The surface lipoproteins of gram-negative bacteria: Protectors and foragers in harsh environments.
Cole et al. (2021)
2 more