6cst

X-ray diffraction
2Å resolution

Structure of human DNA polymerase kappa with DNA

Released:

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase kappa Chains: A, B
Molecule details ›
Chains: A, B
Length: 551 amino acids
Theoretical weight: 63.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UBT6 (Residues: 1-526; Coverage: 61%)
Gene names: DINB1, POLK
Sequence domains:
Structure domains:
DNA (5'-D(P*AP*TP*AP*CP*AP*TP*AP*CP*C)-3') Chains: C, P
Molecule details ›
Chains: C, P
Length: 9 nucleotides
Theoretical weight: 2.68 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*TP*AP*CP*TP*GP*GP*TP*AP*TP*GP*TP*AP*T)-3') Chains: D, T
Molecule details ›
Chains: D, T
Length: 13 nucleotides
Theoretical weight: 4 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 67.579Å b: 124.63Å c: 165.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.198 0.234
Expression systems:
  • Escherichia coli
  • Not provided