X-ray diffraction
2Å resolution

Arg65Gln Mutagenic E.coli PCK

Source organism: Escherichia coli
Entry authors: Sokaribo AS, Cotelesage JH, Novakovski B, Goldie H, Sanders D

Function and Biology Details

Reaction catalysed:
ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO(2)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Phosphoenolpyruvate carboxykinase (ATP) Chain: A
Molecule details ›
Chain: A
Length: 540 amino acids
Theoretical weight: 59.68 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
  • Canonical: P22259 (Residues: 1-540; Coverage: 100%)
Gene names: JW3366, b3403, pck, pckA
Sequence domains: Phosphoenolpyruvate carboxykinase
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: C2
Unit cell:
a: 124.993Å b: 95.301Å c: 46.34Å
α: 90° β: 96.4° γ: 90°
R R work R free
0.16 0.156 0.21
Expression system: Escherichia coli