› 6cpg

X-ray diffraction
2.8Å resolution

Structure of dephosphorylated Aurora A (122-403) in complex with inhibiting monobody and AT9283 in an inactive conformation

Released:
DOI: 10.2210/pdb6cpg/pdb
PDB entry 6cpg coloured by chain, front view.
PDB entry 6cpg coloured by chain, side view.
PDB entry 6cpg coloured by chain, top view.
Source organisms:
Primary publication:
Dynamics of human protein kinase Aurora A linked to drug selectivity.
Pitsawong W, Buosi V, Otten R, Agafonov RV, Zorba A, Kern N, Kutter S, Kern G, Pádua RA, Meniche X, Kern D
Elife 7 (2018)
PMID: 29901437

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127240 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Aurora kinase A Chains: A, D
Molecule details ›
Chains: A, D
Length: 285 amino acids
Theoretical weight: 32.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O14965 (Residues: 122-403; Coverage: 70%)
Gene names: AIK, AIRK1, ARK1, AURA, AURKA, AYK1, BTAK, IAK1, STK15, STK6
Sequence domains: Protein kinase domain
Monobody Chains: B, E
Molecule details ›
Chains: B, E
Length: 93 amino acids
Theoretical weight: 9.85 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand 35R 2 x 35R
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P212121
Unit cell:
a: 63.86Å b: 69.7Å c: 175.56Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.285 0.279 0.335
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Advances in targeted degradation of endogenous proteins.
Röth et al. (2019)
2 more

2 mentions without citation

Dynamics of human protein kinase Aurora A linked to drug selectivity.
Pitsawong et al. (2018)
1 more